Association of stromal cell-derived factor-1-3'A polymorphism to higher mobilization of hematopoietic stem cells CD34+ in Tunisian population

Transplant Proc. 2011 Mar;43(2):635-8. doi: 10.1016/j.transproceed.2011.01.016.

Abstract

We explored the influence of polymorphisms in genes encoding the chemokine stromal cell-derived factor-1 (SDF-1)/CXCL12 in a cohort of Tunisian patients with malignant hematologic diseases multiple myeloma [MM], non-Hodgkin's lymphoma [NHL], Hodgkin's disease, and acute myeloid leukemia [AML], who underwent stem cell mobilization for autologous transplantation versus a group of healthy donors for allogeneic transplantation. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLp) analysis was used for rapid identification of genotypes. Significant associations for SDF1-3'A polymorphism were observed exclusively in patients with MM and NHL. While there was a lack of all association of SDF-1 polymorphism with AML patients. However, considering that the ability of mobilization varies among subjects, we have observed that the SDF1-3'A allele was associated with good mobilization capacity. Interestingly, the association was mainly observed among healthy allogeneic transplant donors where the analysis was not biased by background disease or chemotherapy (P=.010; odds ratio=2.603; confidence interval [95%]=1.239-5.466).

MeSH terms

  • Alleles
  • Antigens, CD34 / biosynthesis*
  • Chemokine CXCL12 / genetics*
  • Hematopoietic Stem Cell Mobilization / methods*
  • Hodgkin Disease / genetics
  • Humans
  • Leukemia, Myeloid, Acute / genetics
  • Lymphoma, Non-Hodgkin / genetics
  • Multiple Myeloma / genetics
  • Odds Ratio
  • Polymerase Chain Reaction / methods
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length
  • Transplantation, Homologous
  • Tunesien

Substances

  • Antigens, CD34
  • Chemokine CXCL12