Abstract
Several plus-strand RNA viruses encode proteins containing macrodomains. These domains possess ADP-ribose-1″-phosphatase (ADRP) activity and/or bind poly(ADP-ribose), poly(A) or poly(G). The relevance of these activities in the viral life cycle has not yet been resolved. Here, we report that genetically engineered mutants of severe acute respiratory syndrome coronavirus (SARS-CoV) and human coronavirus 229E (HCoV-229E) expressing ADRP-deficient macrodomains displayed an increased sensitivity to the antiviral effect of alpha interferon compared with their wild-type counterparts. The data suggest that macrodomain-associated ADRP activities may have a role in viral escape from the innate immune responses of the host.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antiviral Agents / immunology*
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Cell Line
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Coronavirus 229E, Human / chemistry
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Coronavirus 229E, Human / enzymology*
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Coronavirus 229E, Human / genetics
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Coronavirus 229E, Human / immunology
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Coronavirus Infections / genetics
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Coronavirus Infections / immunology*
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Coronavirus Infections / virology
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Humans
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Interferon-alpha / genetics
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Interferon-alpha / immunology*
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Molecular Sequence Data
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Phosphoric Monoester Hydrolases / chemistry*
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Phosphoric Monoester Hydrolases / genetics
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Phosphoric Monoester Hydrolases / immunology
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Protein Structure, Tertiary
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Sequence Alignment
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Severe Acute Respiratory Syndrome / genetics
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Severe Acute Respiratory Syndrome / immunology*
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Severe Acute Respiratory Syndrome / virology
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Severe acute respiratory syndrome-related coronavirus / chemistry
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Severe acute respiratory syndrome-related coronavirus / drug effects
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Severe acute respiratory syndrome-related coronavirus / enzymology*
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Severe acute respiratory syndrome-related coronavirus / genetics
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Viral Proteins / chemistry*
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Viral Proteins / genetics
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Viral Proteins / immunology
Substances
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Antiviral Agents
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Interferon-alpha
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Viral Proteins
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Phosphoric Monoester Hydrolases