The anxiolytic-like profile of the nociceptin receptor agonist, endo-8-[bis(2-chlorophenyl)methyl]-3-phenyl-8-azabicyclo[3.2.1]octane-3-carboxamide (SCH 655842): comparison of efficacy and side effects across rodent species

Eur J Pharmacol. 2011 Jul 1;661(1-3):63-71. doi: 10.1016/j.ejphar.2011.04.034. Epub 2011 Apr 28.

Abstract

The endogenous opioid-like peptide, nociceptin, produces anxiolytic-like effects that are mediated via the nociceptin (NOP) receptor. Similarly, synthetic, non-peptide NOP agonists produce robust anxiolytic-like effects although these effects are limited by marked side effects. In the present studies, the effects of a novel NOP receptor agonist, SCH 655842, were examined in rodent models sensitive to anxiolytic drugs and tests measuring potential adverse affects. Oral administration of SCH 655842 produced robust, anxiolytic-like effects in three species, i.e., rat, guinea pig, and mouse. Specifically, SCH 655842 was effective in rat conditioned lick suppression (3-10 mg/kg) and fear-potentiated startle (3-10 mg/kg) tests, a guinea pig pup vocalization test (1-3 mg/kg), as well as in mouse Geller-Seifter (30 mg/kg) and marble burying (30 mg/kg) tests. The anxiolytic-like effect of SCH 655842 in the conditioned lick suppression test was attenuated by the NOP antagonist, J-113397. In mice, SCH 655842 reduced locomotor activity and body temperature at doses similar to the anxiolytic-like dose and these effects were absent in NOP receptor knockout mice. In rats, SCH 655842 did not produce adverse behavioral effects up to doses of 70-100 mg/kg. Pharmacokinetic studies in the rat confirmed dose-related increases in plasma and brain levels of SCH 655842 across a wide oral dose range. Taken together, SCH 655842 may represent a NOP receptor agonist with improved tolerability compared to other members of this class although further studies are necessary to establish whether this extends to higher species.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Anxiety Agents / adverse effects*
  • Anti-Anxiety Agents / blood
  • Anti-Anxiety Agents / pharmacokinetics
  • Anti-Anxiety Agents / pharmacology*
  • Azabicyclo Compounds / adverse effects*
  • Azabicyclo Compounds / blood
  • Azabicyclo Compounds / pharmacokinetics
  • Azabicyclo Compounds / pharmacology*
  • Behavior, Animal / drug effects
  • Behavior, Animal / physiology
  • Body Temperature / drug effects
  • Brain / drug effects
  • Brain / metabolism
  • Conditioning, Psychological / drug effects
  • Conditioning, Psychological / physiology
  • Dose-Response Relationship, Drug
  • Fear / drug effects
  • Fear / physiology
  • Female
  • Gene Knockout Techniques
  • Guinea Pigs
  • Male
  • Mice
  • Motor Activity / drug effects
  • Nociceptin Receptor
  • Rats
  • Receptors, Opioid / agonists*
  • Receptors, Opioid / deficiency
  • Receptors, Opioid / genetics
  • Rotarod Performance Test
  • Species Specificity
  • Vocalization, Animal / drug effects

Substances

  • Anti-Anxiety Agents
  • Azabicyclo Compounds
  • Receptors, Opioid
  • SCH 655842
  • Nociceptin Receptor