Abstract
Contributions by basophils to allergic and helminth immunity remain incompletely defined. Using sensitive interleukin 4 (Il4) reporter alleles, we demonstrate here that basophil IL-4 production occurs by a CD4(+) T cell-dependent process restricted to the peripheral tissues affected. We genetically marked and achieved specific deletion of basophils and found that basophils did not mediate T helper type 2 (T(H)2) priming in vivo. Two-photon imaging confirmed that basophils did not interact with antigen-specific T cells in lymph nodes but engaged in prolonged serial interactions with T cells in lung tissues. Although targeted deletion of IL-4 and IL-13 in either CD4(+) T cells or basophils had a minimal effect on worm clearance, deletion from both lineages demonstrated a nonredundant role for basophil cytokines in primary helminth immunity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Basophils / immunology*
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Basophils / metabolism
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / metabolism
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Female
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Flow Cytometry
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Helminthiasis, Animal / immunology
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Helminthiasis, Animal / metabolism
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Helminthiasis, Animal / parasitology
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Host-Parasite Interactions / immunology
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Humans
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Interleukin-13 / genetics
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Interleukin-13 / immunology
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Interleukin-13 / metabolism
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Interleukin-4 / genetics
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Interleukin-4 / immunology*
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Interleukin-4 / metabolism
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Liver / immunology
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Liver / metabolism
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Liver / parasitology
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Lung / immunology*
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Lung / metabolism
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Lung / parasitology
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Lung Diseases, Parasitic / immunology
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Lung Diseases, Parasitic / metabolism
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Transgenic
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Microscopy, Fluorescence, Multiphoton
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Nippostrongylus / immunology
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Nippostrongylus / physiology
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Schistosoma mansoni / immunology
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Schistosoma mansoni / physiology
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Strongylida Infections / immunology*
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Strongylida Infections / metabolism
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Strongylida Infections / parasitology
Substances
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Interleukin-13
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Green Fluorescent Proteins
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Interleukin-4