Oxidative DNA damage in early pregnancy and risk of gestational diabetes mellitus: A pilot study

Clin Biochem. 2011 Jul;44(10-11):804-8. doi: 10.1016/j.clinbiochem.2011.04.023. Epub 2011 May 13.

Abstract

Objectives: To examine the association of maternal early pregnancy oxidative stress with risk of gestational diabetes mellitus (GDM).

Design and methods: A pilot prospective, nested case-control study was conducted. Study participants were recruited before 20weeks gestation. Maternal urinary 8-hydroxydeoxyguanosine (8-OHdG), a biomarker of systemic oxidative DNA damage and repair, was measured using competitive immunoassays. Logistic regression was used to calculate odds ratio (OR) and 95% confidence intervals (95%CI).

Results: Elevations in early pregnancy urinary 8-OHdG concentrations were associated with increased GDM risk. After adjusting for confounders, the OR for extreme quartiles (≥8.01 vs. <4.23ng/mg creatinine) of 8-OHdG was 3.79 (95%CI 1.03-14.00). The risk for GDM was highest for overweight women with urine 8-OHdG concentrations ≥8.01ng/mg creatinine (OR=5.36, 95%CI 1.33-21.55) when compared with lean women who had 8-OHdG concentrations <8.01ng/mg creatinine.

Conclusions: Elevated urine 8-OHdG concentrations in early pregnancy appear to be associated with increased GDM risk.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Case-Control Studies
  • Confidence Intervals
  • DNA Damage*
  • Demography
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / urine
  • Diabetes, Gestational / pathology*
  • Diabetes, Gestational / urine
  • Female
  • Humans
  • Odds Ratio
  • Oxidative Stress*
  • Pilot Projects
  • Pregnancy
  • Risk Factors
  • Washington
  • Young Adult

Substances

  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine