Diagnosis of dengue fever in North West Italy in travelers from endemic areas: a retrospective study

J Clin Virol. 2011 Aug;51(4):259-63. doi: 10.1016/j.jcv.2011.05.011. Epub 2011 Jun 1.

Abstract

Background: Domestic outbreaks of Dengue (DENV) fever from imported cases have to be considered a possible risk in non-endemic countries where Dengue vectors are present, such as in Italy.

Objective: To review imported acute/recent DENV infections in a one-year survey in a North West Italy region where the presence of Aedes albopictus is documented.

Study design: We retrospectively reviewed laboratory and clinical records of Italian febrile travelers from Dengue endemic areas referring to the local reference Centre for Infectious Disease, covering a population of about 4 million people.

Results: Acute/recent DENV infection was identified in 15 out of 91 travelers from endemic areas (16.5%) including 12 primary and 3 secondary infections; in 6 patients the virus was detectable in blood according to molecular real-time Polymerase Chain Reaction-based assays: in 9 patients the diagnosis of DENV infection was accomplished by the combination of specific IgM reactivity, high IgG titers, IgG seroconversion from negative to positive and increasing (four-fold) IgG titers in paired serum samples. Two cases of DENV infections were imported from South Egypt in patients travelling together, confirming the importance of returning travelers as sentinels of a rapidly changing epidemiology in specific geographic areas.

Conclusions: Our findings outline the high rate of imported Dengue infection in North West Italy and emphasize the need for a continued Dengue surveillance in non-endemic countries as well as a careful evaluation and follow-up of febrile patients returning from Dengue endemic countries.

MeSH terms

  • Adult
  • Aged
  • Antibodies, Viral / blood
  • Blood / virology
  • Dengue / diagnosis*
  • Dengue / epidemiology*
  • Dengue / immunology
  • Dengue / virology
  • Dengue Virus / genetics
  • Dengue Virus / immunology
  • Dengue Virus / isolation & purification
  • Female
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Italy / epidemiology
  • Male
  • Middle Aged
  • Prevalence
  • RNA, Viral / blood
  • Retrospective Studies
  • Travel*

Substances

  • Antibodies, Viral
  • Immunoglobulin G
  • Immunoglobulin M
  • RNA, Viral