Saliva is a reliable and practical source of germline DNA for genome-wide studies in chronic lymphocytic leukemia

Leuk Res. 2011 Oct;35(10):1419-22. doi: 10.1016/j.leukres.2011.05.024. Epub 2011 Jun 12.

Abstract

High-throughput genomics requires tumor DNA matched to germline DNA, that cannot be easily obtained in the context of leukemia. Using chronic lymphocytic leukemia as a model, saliva DNA was frequently devoid of tumor DNA also during overt disease, and passed quality controls for SNP-array (77/102, 75.4%) and next generation sequencing (71/102, 69.6%). Compared to saliva, urine provides germline DNA of similar quality but in lower amounts. Saliva DNA was successfully run on SNP 6.0 arrays, and passed quality control call rate thresholds. On these bases, saliva represents a useful source of germline DNA for high-throughput genomic studies of hematologic neoplasia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / urine
  • Genome, Human
  • Genome-Wide Association Study / methods*
  • Genomics / methods*
  • High-Throughput Screening Assays
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide
  • Saliva / chemistry*
  • Sequence Analysis, DNA*

Substances

  • DNA