Abstract
Branched RNAs with three- or four-way junctions were designed by assembling single-stranded RNA for RNA interference. Human Dicer transformed branched RNAs into about 20 base pairs of double-stranded RNA, which is a standard siRNA species. Our tetramer design provides a potent silencing effect over a period of 5 days.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DEAD-box RNA Helicases / antagonists & inhibitors
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DEAD-box RNA Helicases / genetics
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DEAD-box RNA Helicases / metabolism
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HeLa Cells
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Humans
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Nanostructures / chemistry*
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RNA Interference*
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RNA, Double-Stranded / chemistry*
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RNA, Double-Stranded / metabolism
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RNA, Small Interfering / metabolism
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Ribonuclease III / antagonists & inhibitors
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Ribonuclease III / genetics
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Ribonuclease III / metabolism
Substances
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RNA, Double-Stranded
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RNA, Small Interfering
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DICER1 protein, human
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Ribonuclease III
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DEAD-box RNA Helicases