Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.)

Mol Biol Rep. 2012 Apr;39(4):3565-72. doi: 10.1007/s11033-011-1130-6. Epub 2011 Jul 1.

Abstract

Late-embryogenesis abundant (LEA) proteins have been reported to be closely correlated with the acquisition of desiccation tolerance during seed development and response of plant to drought, salinity, and freezing, etc. In this study, a LEA gene, CarLEA4 (GenBank accession no. GU247511), was isolated from chickpea based on a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol (PEG). CarLEA4 contained two exons and one intron within genomic DNA sequence and encoded a putative polypeptide of 152 amino acids. CarLEA4 had a conserved pfam domain, and showed high similarity to the group 4 LEA proteins in secondary structure. It was localized in the nucleus. The transcripts of CarLEA4 were detected in many chickpea organs including seedling leaves, stems, roots, flowers, young pods, and young seeds. CarLEA4 was inhibited by leaf age and showed expression changes in expression during seed development, pod development and germination. Furthermore, the expression of CarLEA4 was strongly induced by drought, salt, heat, cold, ABA, IAA, GA(3) and MeJA. Our results suggest that CarLEA4 encodes a protein of LEA group 4 and may be involved in various plant developmental processes and abiotic stress responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Nucleus / metabolism
  • Cicer / genetics*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant
  • Genes, Plant / genetics*
  • Green Fluorescent Proteins / metabolism
  • Molecular Sequence Data
  • Multigene Family / genetics*
  • Organ Specificity / genetics
  • Plant Growth Regulators / pharmacology
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Protein Structure, Tertiary
  • Real-Time Polymerase Chain Reaction
  • Sequence Alignment
  • Stress, Physiological / drug effects
  • Stress, Physiological / genetics

Substances

  • Plant Growth Regulators
  • Plant Proteins
  • late embryogenesis abundant protein, plant
  • Green Fluorescent Proteins

Associated data

  • GENBANK/GU247511