Effect of intracerebroventricular injections of prolactin-releasing peptide on prolactin release and stress-related responses in steers

Anim Sci J. 2011 Apr;82(2):314-9. doi: 10.1111/j.1740-0929.2010.00841.x. Epub 2011 Mar 4.

Abstract

Some evidence suggests that there might be a species difference in the effect of intracerebroventricularly administered (ICV) prolactin-releasing peptide (PrRP) between rodents and sheep. We compared the levels of cortisol (CORT) and prolactin (PRL), rectal temperature (RT) and behavioral responses to ICV bovine PrRP (bPrRP) in steers. ICV bPrRP (0.2, 2 and 20 nmol/200 µL) tended to evoke a dose-related increase in CORT concentrations and 0.2 nmol of bPrRP induced transient increase in PRL concentrations. A significant time-treatment interaction was observed for the percent change of CORT (P<0.05) and PRL (P<0.05) from pre-injection value. The time-treatment interaction for changes in RT was not significant (P=0.50). There tended to be a difference among the four treatments in terms of maximum change in RT from the pre-injection value between 0 and 90 min (P<0.1). Stress-related behavioral signs were not observed in the present experiment. These findings indicate that ICV bPrRP increased CORT and PRL levels, suggesting that central PrRP might participate in controlling the hypothalamo-pituitary-adrenal axis and PRL release in cattle, unlike sheep. In contrast, central PrRP is unlikely to be involved in controlling the behavior of this species because ICV bPrRP did not induce marked changes in their behavior.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal / physiology
  • Body Temperature
  • Cattle / physiology*
  • Dose-Response Relationship, Drug
  • Hydrocortisone / blood
  • Injections, Intraventricular
  • Male
  • Prolactin / metabolism*
  • Prolactin-Releasing Hormone / administration & dosage*
  • Rectum / physiology
  • Stress, Psychological / physiopathology

Substances

  • Prolactin-Releasing Hormone
  • Prolactin
  • Hydrocortisone