Abstract
Brazil represents the greatest beef producer among tropical countries, and the major obstacle for meat international trade is sanitary problems especially closely related to viral encephalitis. The goal of this study was to estimate the accuracy of the glycol and US9 gene-based polymerase chain reactions (PCRs) for the detection of bovine Herpesvirus type 5 (BoHV-5) from decomposed brain samples (n = 95). For this purpose, a latent-class (bayesian) approach was used. Sensitivity (Se) was estimated to be 70% (95% probability interval, 40-80) and specificity (Sp) 100% in the statistical analysis for both PCRs used. Accordingly, a minimum of ≥40% of the calves was estimated to harbor BoHV-5 DNA even after 72 h of decomposition at room temperature. It was concluded that US9 gene-based PCR could also be considered a cost-effective alternative in sanitary programmers. However, given the importance of veterinary diagnoses, PCR-positive samples should be further confirmed through in vitro isolation and/or sequencing.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Abattoirs
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Animals
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Bayes Theorem
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Brain / virology*
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Brazil / epidemiology
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Cattle
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Cattle Diseases / diagnosis*
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Cattle Diseases / epidemiology
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Cattle Diseases / virology
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DNA, Viral / analysis
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Encephalitis, Viral / diagnosis
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Encephalitis, Viral / epidemiology
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Encephalitis, Viral / veterinary*
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Encephalitis, Viral / virology
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Herpesviridae Infections / diagnosis
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Herpesviridae Infections / epidemiology
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Herpesviridae Infections / veterinary*
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Herpesviridae Infections / virology
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Herpesvirus 5, Bovine / genetics
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Herpesvirus 5, Bovine / isolation & purification*
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Male
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Meningoencephalitis / diagnosis
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Meningoencephalitis / epidemiology
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Meningoencephalitis / veterinary*
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Meningoencephalitis / virology
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / veterinary
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Prevalence
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Sensitivity and Specificity
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Viral Envelope Proteins / genetics
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Viral Matrix Proteins / genetics
Substances
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DNA, Viral
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Viral Envelope Proteins
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Viral Matrix Proteins