MicroRNA-22 and microRNA-140 suppress NF-κB activity by regulating the expression of NF-κB coactivators

Biochem Biophys Res Commun. 2011 Aug 12;411(4):826-31. doi: 10.1016/j.bbrc.2011.07.048. Epub 2011 Jul 21.

Abstract

Nuclear factor κB (NF-κB) is a transcription factor that regulates a set of genes that are critical to many biological phenomena, including liver tumorigenesis. To identify microRNAs (miRNAs) that regulate NF-κB activity in the liver, we screened 60 miRNAs expressed in hepatocytes for their ability to modulate NF-κB activity. We found that miRNA-22 and miRNA-140-3p significantly suppressed NF-κB activity by regulating the expression of nuclear receptor coactivator 1 (NCOA1) and nuclear receptor-interacting protein 1 (NRIP1), both of which are NF-κB coactivators. Our results provide new information about the roles of miRNAs in the regulation of NF-κB activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics*
  • Base Sequence
  • Cell Line
  • Gene Expression Regulation*
  • Genes, Reporter
  • Hepatocytes / metabolism
  • Humans
  • Liver / metabolism*
  • Luciferases / genetics
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • NF-kappa B / antagonists & inhibitors*
  • NF-kappa B / metabolism
  • Nuclear Proteins / genetics*
  • Nuclear Receptor Coactivator 1 / genetics*
  • Nuclear Receptor Interacting Protein 1
  • Promoter Regions, Genetic

Substances

  • Adaptor Proteins, Signal Transducing
  • MIRN22 microRNA, human
  • MicroRNAs
  • Mirn140 microRNA, human
  • NF-kappa B
  • NRIP1 protein, human
  • Nuclear Proteins
  • Nuclear Receptor Interacting Protein 1
  • Luciferases
  • NCOA1 protein, human
  • Nuclear Receptor Coactivator 1