Abstract
Interleukin 10 (IL-10) is a multifunctional cytokine that regulates diverse functions of immune cells. Natural killer (NK) cells express the IL-10 and IL-10 receptor, but little is known about the function of IL-10 on NK cell activation. In this study, we show the expression and role of IL-10 in human NK cells. Among the cytokines tested, IL-15 was the most potent inducer of IL-10, with a maximal peak expression at 5 h after treatment. Furthermore, IL-10 receptor was shown to be expressed in NK cells. IL-10 alone had a significant effect on NK cytotoxicity which additively increased NK cell cytotoxicity in the presence of IL-15. Neutralizing IL-10 with anti-IL-10 antibody suppressed the inductive effect of IL-10 on NK cell cytotoxicity; however, IL-10 had no effect on IFN-γ or TNF-α production or NK cell activatory receptor expression. STAT signals are implicated as a key mediator of IL-10/IL-15 cytotoxicity response. Thus, the effect of IL-10 on NK cells is particularly interesting with regard to the STAT3 signal that was enhanced by IL-10 or IL-15.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Neutralizing / immunology
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Antibodies, Neutralizing / pharmacology
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Cell Line
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Cell Proliferation / drug effects
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Cytotoxicity, Immunologic / drug effects*
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Enzyme-Linked Immunosorbent Assay
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Flow Cytometry
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Humans
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Interferon-gamma / immunology
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Interleukin-10* / antagonists & inhibitors
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Interleukin-10* / immunology
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Interleukin-10* / metabolism
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Interleukin-15 / immunology*
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Interleukin-15 / metabolism
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Interleukin-15 / pharmacology
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Killer Cells, Natural / drug effects
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Killer Cells, Natural / immunology*
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Killer Cells, Natural / metabolism
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Lymphocyte Activation / drug effects
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Lymphocyte Activation / immunology
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Receptors, Interleukin-10 / immunology*
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Receptors, Interleukin-10 / metabolism
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STAT3 Transcription Factor / genetics
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STAT3 Transcription Factor / metabolism
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Signal Transduction / immunology*
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Tumor Necrosis Factor-alpha / immunology
Substances
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Antibodies, Neutralizing
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Interleukin-15
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Receptors, Interleukin-10
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STAT3 Transcription Factor
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STAT3 protein, human
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Tumor Necrosis Factor-alpha
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Interleukin-10
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Interferon-gamma