Abstract
No disease-specific neuronal antibodies have so far been defined in neuro-Behçet's disease (NBD). Immunohistochemistry and immunocytochemistry studies showed antibodies to hippocampal and cerebellar molecular layers and the surface antigens of cultured hippocampal neurons in sera and/or cerebrospinal fluids (CSF) of 13 of 20 NBD and 6 of 20 BD patients but not in multiple sclerosis or headache controls. Screening with a protein macroarray led to identification of stress-induced-phosphoprotein-1 (STIP-1) as an antigenic target. High-titer STIP-1-antibodies were detected in 6 NBD patients' sera but not in controls. These results suggest that neuronal antibodies could be useful as diagnostic biomarkers in NBD.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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Autoantibodies / biosynthesis*
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Behcet Syndrome / immunology*
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Behcet Syndrome / metabolism
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Behcet Syndrome / pathology
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Female
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Heat-Shock Proteins / blood
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Heat-Shock Proteins / immunology*
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Hippocampus / blood supply
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Hippocampus / immunology
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Hippocampus / pathology
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Humans
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Male
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Mannose-Binding Protein-Associated Serine Proteases / physiology
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Middle Aged
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Multiple Sclerosis / immunology
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Multiple Sclerosis / metabolism
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Multiple Sclerosis / pathology
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Nerve Degeneration / immunology*
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Nerve Degeneration / metabolism
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Nerve Degeneration / pathology
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Neurons / immunology*
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Neurons / metabolism
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Neurons / pathology
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Organ Specificity / immunology
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Rats
Substances
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Autoantibodies
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Heat-Shock Proteins
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STIP1 protein, human
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MASP2 protein, human
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Mannose-Binding Protein-Associated Serine Proteases