[Advances of development of phage display systems]

Yi Chuan. 2011 Oct;33(10):1113-20. doi: 10.3724/sp.j.1005.2011.01113.
[Article in Chinese]

Abstract

The phage display technology (PDT) was unique in genetic engineering and recombinant expression. The phage display systems (PDS) were platforms (kits) composed of genetic modified phages, helper phages, and host bacteria. This review concisely summarized the development of four types of PDS, based on M13, λ, T4, and T7 phages, in terms of phage molecular genetics and genetic (gene or genome) engineering. We addressed on the key components and their genetic (genomic) engineering for modifications, the technical features of different anchors, and the development progress and selection reference of those different kits.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacteriophage M13 / genetics
  • Bacteriophage T4 / genetics
  • Bacteriophage T7 / genetics
  • Bacteriophage lambda / genetics
  • Genetic Engineering*
  • Peptide Library*

Substances

  • Peptide Library