Abstract
The DNA binding subunit of the transcription factor NF-kappa B, p50, has been cloned. p50 appears to be synthesized as a larger protein that is then processed to its functional size. Sequence analysis reveals remarkable homology for over 300 amino acids at the amino-terminal end to the oncogene v-rel, its cellular homolog c-rel, and the Drosophila maternal effect gene dorsal. This establishes NF-kappa B as a member of the rel family of proteins, all of which display nuclear-cytosolic translocation. Protein sequence from the p65 polypeptide has established that it is not encoded in the same mRNA as p50. However, p65 appears homologous to c-rel, suggesting that c-rel may form heterodimers with p50 and rel may include a homodimerization motif.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Cloning, Molecular / methods
-
DNA / genetics
-
DNA / isolation & purification
-
DNA-Binding Proteins / genetics*
-
DNA-Binding Proteins / isolation & purification
-
Drosophila / genetics*
-
Female
-
Genes*
-
Humans
-
Lung / metabolism
-
Macromolecular Substances
-
Mice
-
Molecular Sequence Data
-
NF-kappa B
-
Oncogene Proteins v-rel
-
Peptide Fragments / isolation & purification
-
Protein Biosynthesis
-
Protein-Tyrosine Kinases / genetics
-
Rabbits
-
Recombinant Proteins / isolation & purification
-
Retroviridae Proteins, Oncogenic / genetics*
-
Sequence Homology, Nucleic Acid
-
Transcription Factors / genetics*
-
Transcription Factors / isolation & purification
-
Transcription, Genetic
-
Trypsin
Substances
-
DNA-Binding Proteins
-
Macromolecular Substances
-
NF-kappa B
-
Oncogene Proteins v-rel
-
Peptide Fragments
-
Recombinant Proteins
-
Retroviridae Proteins, Oncogenic
-
Transcription Factors
-
DNA
-
Protein-Tyrosine Kinases
-
Trypsin
Associated data
-
GENBANK/M37732
-
GENBANK/M57999