Effects of DPP-4 inhibitors on the heart in a rat model of uremic cardiomyopathy

PLoS One. 2011;6(11):e27861. doi: 10.1371/journal.pone.0027861. Epub 2011 Nov 18.

Abstract

Background: Uremic cardiomyopathy contributes substantially to mortality in chronic kidney disease (CKD) patients. Glucagon-like peptide-1 (GLP-1) may improve cardiac function, but is mainly degraded by dipeptidyl peptidase-4 (DPP-4).

Methodology/principal findings: In a rat model of chronic renal failure, 5/6-nephrectomized [5/6N] rats were treated orally with DPP-4 inhibitors (linagliptin, sitagliptin, alogliptin) or placebo once daily for 4 days from 8 weeks after surgery, to identify the most appropriate treatment for cardiac dysfunction associated with CKD. Linagliptin showed no significant change in blood level AUC(0-∞) in 5/6N rats, but sitagliptin and alogliptin had significantly higher AUC(0-∞) values; 41% and 28% (p = 0.0001 and p = 0.0324), respectively. No correlation of markers of renal tubular and glomerular function with AUC was observed for linagliptin, which required no dose adjustment in uremic rats. Linagliptin 7 µmol/kg caused a 2-fold increase in GLP-1 (AUC 201.0 ng/l*h) in 5/6N rats compared with sham-treated rats (AUC 108.6 ng/l*h) (p = 0.01). The mRNA levels of heart tissue fibrosis markers were all significantly increased in 5/6N vs control rats and reduced/normalized by linagliptin.

Conclusions/significance: DPP-4 inhibition increases plasma GLP-1 levels, particularly in uremia, and reduces expression of cardiac mRNA levels of matrix proteins and B-type natriuretic peptides (BNP). Linagliptin may offer a unique approach for treating uremic cardiomyopathy in CKD patients, with no need for dose-adjustment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Area Under Curve
  • Cardiomyopathies / etiology
  • Cardiomyopathies / physiopathology
  • Cardiomyopathies / prevention & control*
  • Dipeptidyl Peptidase 4 / metabolism
  • Dipeptidyl-Peptidase IV Inhibitors / pharmacokinetics
  • Dipeptidyl-Peptidase IV Inhibitors / pharmacology*
  • Disease Models, Animal*
  • Gene Expression Regulation / drug effects
  • Glomerular Filtration Rate
  • Glucagon-Like Peptide 1 / blood
  • Glucagon-Like Peptide 1 / metabolism
  • Heart / drug effects*
  • Heart / physiopathology
  • Humans
  • Kidney Failure, Chronic / complications
  • Kidney Failure, Chronic / physiopathology
  • Kidney Failure, Chronic / prevention & control
  • Linagliptin
  • Myocardium / metabolism
  • Natriuretic Peptide, Brain / genetics
  • Nephrectomy
  • Piperidines / pharmacokinetics
  • Piperidines / pharmacology
  • Purines / pharmacokinetics
  • Purines / pharmacology
  • Pyrazines / pharmacokinetics
  • Pyrazines / pharmacology
  • Quinazolines / pharmacokinetics
  • Quinazolines / pharmacology
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sitagliptin Phosphate
  • Triazoles / pharmacokinetics
  • Triazoles / pharmacology
  • Uracil / analogs & derivatives
  • Uracil / pharmacokinetics
  • Uracil / pharmacology
  • Uremia / complications
  • Uremia / physiopathology
  • Uremia / prevention & control

Substances

  • Dipeptidyl-Peptidase IV Inhibitors
  • Piperidines
  • Purines
  • Pyrazines
  • Quinazolines
  • Triazoles
  • Natriuretic Peptide, Brain
  • Linagliptin
  • Uracil
  • Glucagon-Like Peptide 1
  • Dipeptidyl Peptidase 4
  • alogliptin
  • Sitagliptin Phosphate