Long noncoding RNA-mediated anti-apoptotic activity in murine erythroid terminal differentiation

Genes Dev. 2011 Dec 15;25(24):2573-8. doi: 10.1101/gad.178780.111. Epub 2011 Dec 8.

Abstract

Long noncoding RNAs (lncRNAs) are differentially expressed under both normal and pathological conditions, implying that they may play important biological functions. Here we examined the expression of lncRNAs during erythropoiesis and identified an erythroid-specific lncRNA with anti-apoptotic activity. Inhibition of this lncRNA blocks erythroid differentiation and promotes apoptosis. Conversely, ectopic expression of this lncRNA can inhibit apoptosis in mouse erythroid cells. This lncRNA represses expression of Pycard, a proapoptotic gene, explaining in part the inhibition of programmed cell death. These findings reveal a novel layer of regulation of cell differentiation and apoptosis by a lncRNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis Regulatory Proteins
  • Apoptosis* / genetics
  • CARD Signaling Adaptor Proteins
  • Cell Differentiation*
  • Cells, Cultured
  • Cytoskeletal Proteins / metabolism
  • Erythroid Cells / cytology*
  • Erythroid Cells / metabolism
  • Erythropoiesis / genetics*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • Gene Knockdown Techniques
  • HEK293 Cells
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • RNA, Untranslated / metabolism*

Substances

  • Apoptosis Regulatory Proteins
  • CARD Signaling Adaptor Proteins
  • Cytoskeletal Proteins
  • Pycard protein, mouse
  • RNA, Untranslated