Abstract
Simultaneous activation of signaling pathways requires dynamic assembly of higher-order protein complexes at the cytoplasmic domains of membrane-associated receptors in a stimulus-specific manner. Here, using the paradigm of cellular activation through cytokine and innate immune receptors, we demonstrate the proof-of-principle application of small molecule probes for the dissection of receptor-proximal signaling processes, such as activation of the transcription factor NF-κB and the protein kinase p38.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Immunity, Innate / drug effects
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Lipopolysaccharides / immunology
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Macrophages / drug effects
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Macrophages / immunology
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Mice
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Mice, Inbred C57BL
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NF-kappa B / immunology*
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Signal Transduction / drug effects*
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Small Molecule Libraries / chemistry
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Small Molecule Libraries / pharmacology*
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p38 Mitogen-Activated Protein Kinases / immunology*
Substances
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Lipopolysaccharides
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NF-kappa B
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Small Molecule Libraries
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p38 Mitogen-Activated Protein Kinases