Abstract
Effects of protein kinase C (PKC) inhibitors and "down-regulation" on insulin and PMA-stimulated 2-deoxyglucose transport were determined in isolated rat adipocytes or BC3H-1 myocytes. In both model systems, H-7, sangivamycin, and staurosporine, inhibitors of the catalytic domain of PKC, each effectively blocked insulin and PMA-stimulated hexose uptake at similar concentrations. In the myocytes, staurosporine completely blocked the insulin effect retained post-chronic phorbol myristate acetate (PMA)-induced "down-regulation." These findings indicate (1) that chronic pretreatment with PMA may not lead to a complete loss of PKC activity in the myocyte, and (2) that PKC is involved in insulin-stimulated hexose transport in both isolated rat adipocytes and BC3H-1 myocytes.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Adipose Tissue / cytology
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Adipose Tissue / enzymology*
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Alkaloids / pharmacology
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Animals
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Biological Transport / drug effects
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Cell Line
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Cell Separation
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Deoxyglucose / pharmacokinetics
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Hexoses / pharmacokinetics*
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Insulin / pharmacokinetics*
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Isoquinolines / pharmacology
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Male
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Muscles / cytology
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Muscles / metabolism*
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Piperazines / pharmacology
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Protein Kinase C / antagonists & inhibitors*
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Pyrimidine Nucleosides / pharmacology
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Rats
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Rats, Inbred Strains
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Staurosporine
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Tetradecanoylphorbol Acetate / pharmacology*
Substances
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Alkaloids
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Hexoses
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Insulin
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Isoquinolines
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Piperazines
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Pyrimidine Nucleosides
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sangivamycin
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Deoxyglucose
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Protein Kinase C
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Staurosporine
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Tetradecanoylphorbol Acetate