Detection of influenza A virus in live bird markets in Kenya, 2009-2011

Influenza Other Respir Viruses. 2013 Mar;7(2):113-9. doi: 10.1111/j.1750-2659.2012.00365.x. Epub 2012 Apr 19.

Abstract

Background: Surveillance for influenza viruses within live bird markets (LBMs) has been recognized as an effective tool for detecting circulating avian influenza viruses (AIVs). In Sub-Saharan Africa, limited data exist on AIVs in animal hosts, and in Kenya the presence of influenza virus in animal hosts has not been described.

Objectives: This surveillance project aimed to detect influenza A virus in poultry traded in five LBMs in Kenya.

Methods: We visited each market monthly and collected oropharyngeal and cloacal specimens from poultry and environmental specimens for virological testing for influenza A by real time RT-PCR. On each visit, we collected information on the number and types of birds in each market, health status of the birds, and market practices.

Results: During March 24, 2009-February 28, 2011, we collected 5221 cloacal and oropharyngeal swabs. Of the 5199 (99·6%) specimens tested, influenza A virus was detected in 42 (0·8%), including 35/4166 (0·8%) specimens from chickens, 3/381 (0·8%) from turkeys, and 4/335 (1·2%) from geese. None of the 317 duck specimens were positive. Influenza was more commonly detected in oropharyngeal [33 (1·3%)] than in cloacal [9 (0·4%)] specimens. None of the 485 environmental specimens were positive. Virus was detected in all five markets during most (14/22) of the months. Ducks and geese were kept longer at the market (median 30 days) than chickens (median 2days).

Conclusions: Influenza A was detected in a small percentage of poultry traded in LBMs in Kenya. Efforts should be made to promote practices that could limit the maintenance and transmission of AIVs in LBMs.

MeSH terms

  • Animals
  • Cloaca / virology
  • Environmental Microbiology
  • Humans
  • Influenza A virus / isolation & purification*
  • Influenza in Birds / epidemiology*
  • Influenza in Birds / virology
  • Kenya / epidemiology
  • Oropharynx / virology
  • Poultry
  • Prevalence
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction