Abstract
Arginine-grafted bio-reducible poly(disulfide amine) (ABP) was incorporated into the poly(amido amine) (PAMAM) dendrimer, creating a high molecular weight bio-reducible polymer, PAM-ABP, to overcome the limitations of the low molecular weight ABP. The newly synthesized PAM-ABP was studied to determine its efficacy as a gene delivery carrier. The PAM-ABP demonstrated superior condensing ability for plasmid DNA through the formation of compact nanosized polyplexes. These compact polyplexes enhanced cellular uptake and were less susceptible to reducing agents, resulting in greater transfection efficiency compared to ABP alone. Based on these results, this newly developed PAM-ABP polyplex is a promising delivery system for clinical gene therapy.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Arginine / administration & dosage*
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Arginine / chemistry
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Benzoxazoles / administration & dosage
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Benzoxazoles / chemistry
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Buthionine Sulfoximine
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Cell Line
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Cell Survival
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DNA / administration & dosage
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DNA / chemistry
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Dendrimers / administration & dosage*
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Dendrimers / chemistry
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Green Fluorescent Proteins / administration & dosage
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Green Fluorescent Proteins / chemistry
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Green Fluorescent Proteins / genetics
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HeLa Cells
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Humans
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Mice
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NIH 3T3 Cells
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Plasmids
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Polyamines / administration & dosage*
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Polyamines / chemistry
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Quinolinium Compounds / administration & dosage
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Quinolinium Compounds / chemistry
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Transfection / methods*
Substances
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Benzoxazoles
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Dendrimers
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PAMAM Starburst
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Polyamines
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Quinolinium Compounds
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arginine-grafted bioreducible poly(disulfide amine)polymer
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1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium
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Green Fluorescent Proteins
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Buthionine Sulfoximine
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DNA
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Arginine