Hybridization-triggered isothermal signal amplification coupled with MutS for label-free and sensitive fluorescent assay of SNPs

Anli Jiao; Jing Zheng; Yaping Hu; Guizhi Zhu; Jishan Li; Huimin Li; Ronghua Yang; Weihong Tan

doi:10.1039/c2cc30882g

Hybridization-triggered isothermal signal amplification coupled with MutS for label-free and sensitive fluorescent assay of SNPs

Chem Commun (Camb). 2012 Jun 7;48(45):5659-61. doi: 10.1039/c2cc30882g. Epub 2012 May 1.

Authors

Anli Jiao¹, Jing Zheng, Yaping Hu, Guizhi Zhu, Jishan Li, Huimin Li, Ronghua Yang, Weihong Tan

Affiliation

¹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and College of Materials and Engineering, Hunan University, Changsha, 410082, PR China.

PMID: 22549134
DOI: 10.1039/c2cc30882g

Abstract

Combining the specific recognization of MutS protein for mismatched DNA sequences with the target-driven molecular switch that acts as both the template and primer of the polymerization reaction, a new label-free and sensitive fluorescent assay strategy for specific single-stranded DNA sequences or SNPs is proposed.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Benzothiazoles
DNA / genetics*
DNA / metabolism
Diamines
Fluorescent Dyes / analysis
MutS DNA Mismatch-Binding Protein / metabolism*
Nucleic Acid Hybridization / methods*
Organic Chemicals / analysis
Polymorphism, Single Nucleotide*
Quinolines
Sensitivity and Specificity
Spectrometry, Fluorescence / methods*

Substances

Benzothiazoles
Diamines
Fluorescent Dyes
Organic Chemicals
Quinolines
SYBR Green I
DNA
MutS DNA Mismatch-Binding Protein