Vps18 deficiency inhibits dendritogenesis in Purkinje cells by blocking the lysosomal degradation of Lysyl Oxidase

Biochem Biophys Res Commun. 2012 Jul 13;423(4):715-20. doi: 10.1016/j.bbrc.2012.06.021. Epub 2012 Jun 12.

Abstract

Dendrite development occupies a central position in the formation of nervous system. However, whether lysosomal degradative function is required for dendritogenesis of neurons remains unknown. We have recently demonstrated the critical role of Vps18 in the lysosomal degradation pathway in mice. Here, we report that Vps18 deficiency severely blocks the dendrite development of Pukinje cells but not cerebral cortical neurons. Furthermore, we also demonstrate that the lysyl oxidase (Lox) protein is degraded through lysosome and accumulated in the Vps18 deficient cerebellum but not in cerebral cortices. Our results suggest that lysosome regulates dendritogenesis of Purkinje cells though degrading Lox.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cerebellum / cytology
  • Cerebellum / enzymology
  • Cerebral Cortex / enzymology
  • Dendrites / enzymology
  • Dendrites / physiology*
  • Lysosomes / enzymology
  • Mice
  • Mice, Knockout
  • Protein-Lysine 6-Oxidase / metabolism*
  • Proteolysis
  • Purkinje Cells / enzymology
  • Purkinje Cells / physiology*
  • Vesicular Transport Proteins / genetics
  • Vesicular Transport Proteins / physiology*

Substances

  • Vesicular Transport Proteins
  • Vps18 protein, mouse
  • Protein-Lysine 6-Oxidase