Abstract
Bacteroides fragilis is found among the normal intestinal flora and is involved in host immunostimulation via TLR2. Its cell surface components, such as LPS and capsular polysaccharides, were reported to participate in host immunostimulation. In this study, we report on the existence of a lipoprotein that acts as a TLR2 stimulant in B. fragilis. The TLR2-stimulating lipoprotein was obtained using Triton X-114-water phase partitioning followed by preparative SDS-PAGE. Its N-terminal hydrophobic peptide, which was separated from a tryptic digest, was characterized as a triacylated lipopeptide, and the lipoprotein was identified as BF1333 by mass spectrometry of Asp-N-digested peptides. These results showed that the lipoprotein acts as a TLR2-stimulating component in B. fragilis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acylation
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Animals
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Antigens, Bacterial / chemistry
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Antigens, Bacterial / immunology*
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Antigens, Bacterial / isolation & purification
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Bacteroides fragilis / immunology*
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Electrophoresis, Polyacrylamide Gel
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Immunization
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Lipoproteins / chemistry
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Lipoproteins / immunology*
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Lipoproteins / isolation & purification
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Macrophages, Peritoneal / immunology*
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Male
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Mass Spectrometry
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Mice
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Mice, Inbred BALB C
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Octoxynol
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Peptide Fragments / chemistry
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Peptide Fragments / immunology*
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Peptide Fragments / isolation & purification
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Polyethylene Glycols / chemistry
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Toll-Like Receptor 2 / immunology
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Toll-Like Receptor 2 / metabolism*
Substances
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Antigens, Bacterial
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Lipoproteins
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Peptide Fragments
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Toll-Like Receptor 2
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Polyethylene Glycols
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Octoxynol
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Nonidet P-40