Including osteoprotegerin and collagen IV in a score-based blood test for liver fibrosis increases diagnostic accuracy

Clin Chim Acta. 2013 Jan 16:415:63-8. doi: 10.1016/j.cca.2012.09.020. Epub 2012 Sep 27.

Abstract

Background: Noninvasive methods for liver fibrosis evaluation in chronic liver diseases have been recently developed, i.e. transient elastography (Fibroscan™) and blood tests (Fibrometer®, Fibrotest®, and Hepascore®). In this study, we aimed to design a new score in chronic hepatitis C (CHC) by selecting blood markers in a large panel and we compared its diagnostic performance with those of other noninvasive methods.

Methods: Sixteen blood tests were performed in 306 untreated CHC patients included in a multicenter prospective study (ANRS HC EP 23 Fibrostar) using METAVIR histological fibrosis stage as reference. The new score was constructed by non linear regression using the most accurate biomarkers.

Results: Five markers (alpha-2-macroglobulin, apolipoprotein-A1, AST, collagen IV and osteoprotegerin) were included in the new function called Coopscore©. Using the Obuchowski Index, Coopscore© shows higher diagnostic performances than for Fibrometer®, Fibrotest®, Hepascore® and Fibroscan™ in CHC. Association between Fibroscan™ and Coopscore© might avoid 68% of liver biopsies for the diagnosis of significant fibrosis.

Conclusion: Coopscore© provides higher accuracy than other noninvasive methods for the diagnosis of liver fibrosis in CHC. The association of Coopscore© with Fibroscan™ increases its predictive value.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Apolipoprotein A-I / blood
  • Aspartate Aminotransferases / blood
  • Biomarkers / blood
  • Collagen Type IV / blood*
  • Female
  • Hepacivirus*
  • Hepatitis C, Chronic / blood
  • Hepatitis C, Chronic / diagnosis*
  • Hepatitis C, Chronic / pathology
  • Hepatitis C, Chronic / virology
  • Humans
  • Liver Cirrhosis / blood
  • Liver Cirrhosis / diagnosis*
  • Liver Cirrhosis / pathology
  • Male
  • Middle Aged
  • Osteoprotegerin / blood*
  • Prospective Studies
  • Research Design
  • Sensitivity and Specificity
  • Severity of Illness Index
  • alpha-Macroglobulins / analysis

Substances

  • A2M protein, human
  • Apolipoprotein A-I
  • Biomarkers
  • Collagen Type IV
  • Osteoprotegerin
  • TNFRSF11B protein, human
  • alpha-Macroglobulins
  • Aspartate Aminotransferases