A DNA based five-state switch with programmed reversibility

Chem Commun (Camb). 2012 Nov 21;48(90):11088-90. doi: 10.1039/c2cc35799b. Epub 2012 Oct 9.

Abstract

A programmable switch based on a DNA hairpin loop is functionalised with a rigid or flexible porphyrin or FAM and TAMRA FRET pair, which provides insight into the restructuring of the hairpin as well as porphyrin-porphyrin coupling. The switch contains five discrete states which can be accessed independently and followed by real-time spectroscopy, opening the way to a quinary computing code.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • Fluorescence Resonance Energy Transfer
  • Inverted Repeat Sequences
  • Porphyrins / chemistry
  • Rhodamines / chemistry

Substances

  • 5-carboxytetramethylrhodamine succinimidyl ester
  • Porphyrins
  • Rhodamines
  • DNA