Cholinesterase inhibitory constituents from Ficus bengalensis

J Asian Nat Prod Res. 2012;14(12):1149-55. doi: 10.1080/10286020.2012.733702. Epub 2012 Oct 29.

Abstract

Bengalensinone (22β-hydroxylup-12,20-dien-3-one; 1), a new lupane triterpene, and benganoic acid (2), a new apocarotenoid, together with lupanyl acetate, 3-acetoxy-9(11),12-ursandiene, stigmasterol, alpinumisoflavone, 4-hydroxyacetophenone, 4-hydroxybenzoic acid, 4-hydroxymellein, and p-coumeric acid were isolated from the methanolic extract of the aerial roots of Ficus bengalensis. The structures of these compounds were established by the combination of 1D NMR (¹H and ¹³C NMR) and 2D NMR (HMQC, HMBC, and COSY) analyses, and mass spectrometry (EI-MS and HR-EI-MS), and in comparison with literature data of the related compounds. Compounds 1 and 2 displayed inhibitory potential against enzyme cholinesterase in a concentration-dependent manner with IC₅₀ values 194.5 and 154.5 μM for acetylcholinesterase and 224.9 and 120.0 μM for butyrycholinesterase, respectively.

MeSH terms

  • Acetophenones / chemistry
  • Acetophenones / isolation & purification
  • Acetophenones / pharmacology
  • Cholinesterase Inhibitors / chemistry
  • Cholinesterase Inhibitors / isolation & purification*
  • Cholinesterase Inhibitors / pharmacology*
  • Dose-Response Relationship, Drug
  • Ficus / chemistry*
  • Inhibitory Concentration 50
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Pakistan
  • Plant Roots / chemistry
  • Triterpenes / chemistry
  • Triterpenes / isolation & purification*
  • Triterpenes / pharmacology*

Substances

  • Acetophenones
  • Cholinesterase Inhibitors
  • Triterpenes
  • bengalensinone
  • lupane
  • 4-hydroxyacetophenone