High CCR5 density on central memory CD4+ T cells in acute HIV-1 infection is mostly associated with rapid disease progression

PLoS One. 2012;7(11):e49526. doi: 10.1371/journal.pone.0049526. Epub 2012 Nov 21.

Abstract

CD4+ central memory T cells play a critical role in the pathogenesis of simian immunodeficiency virus disease, and the CCR5 density on the surface of CD4 T cells is an important factor in human immunodeficiency virus (HIV)-1 disease progression. We hypothesized that quantifying central memory cells and CCR5 expression in the early stages of HIV-infection could provide useful prognostic information. We enrolled two different groups of acute HIV-infected subjects. One group progressed to CD4 T cell numbers below 250 cells/µl within 2 years (CD4 Low group), while the other group maintained CD4 cell counts above 450 cells/µl over 2 years (CD4 High group). We compared the CCR5 levels and percentage of CD4 subsets between the two groups during the 1st year of HIV infection. We found no differences between the two groups regarding the percentage of naïve, central memory and effector memory subsets of CD4 cells during the 1st year of HIV-1 infection. CCR5 levels on CD4+ CM subset was higher in the CD4 Low group compared with the CD4 High group during the 1st year of HIV-1 infection. High CCR5 levels on CD4 central memory cells in acute HIV infection are mostly associated with rapid disease progression. Our data suggest that low CCR5 expression on CD4 central memory cells protects CD4 cells from direct virus infection and favors the preservation of CD4(+) T cell homeostasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Monoclonal / chemistry
  • CD4 Lymphocyte Count
  • CD4-Positive T-Lymphocytes / cytology*
  • Cell Separation
  • Cohort Studies
  • Disease Progression
  • Flow Cytometry
  • HIV Antigens / chemistry
  • HIV Antigens / metabolism*
  • HIV-1 / metabolism*
  • Humans
  • Male
  • Middle Aged
  • Real-Time Polymerase Chain Reaction / methods
  • Receptors, CCR5 / chemistry
  • Receptors, CCR5 / metabolism*

Substances

  • Antibodies, Monoclonal
  • HIV Antigens
  • Receptors, CCR5

Grants and funding

This study was supported in part by the National 12th Five-Year Major Projects of China (2012ZX10001-003, 2012ZX10001-006), the Beijing Municipal of Science and Technology Major Project (D09050703590901), the National Natural Science Foundation of China (81101250), Capital Health in the Development of Research and Special Projiect of China(2011–2018-02) and the Beijing Key Laboratory (BZ0089). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.