A multi-biomarker risk score improves prediction of long-term mortality in patients with advanced heart failure

Int J Cardiol. 2013 Sep 30;168(2):1251-7. doi: 10.1016/j.ijcard.2012.11.052. Epub 2012 Dec 4.

Abstract

Background: Accurate risk prediction is important for an adequate management of heart failure (HF) patients. We assessed the incremental prognostic ability of a multi-biomarker approach in advanced HF.

Methods: In 349 patients with advanced HF (median 75 years, 66% male) we investigated the incremental prognostic value of 12 novel biomarkers involved in different pathophysiological pathways including inflammation, immunological activation, oxidative stress, cell growth, remodeling, angiogenesis and apoptosis.

Results: During a median follow-up of 4.9 years 55.9% of patients died. Using multivariable Cox regression and bootstrap variable-selection age, chronic obstructive pulmonary disease, N-terminal pro-B-type natriuretic peptide (NT-proBNP) and the following 5 novel biomarkers were retained in the best mortality prediction model: the chemokine fractalkine, the angiogenic and mitogenic hepatocyte growth factor (HGF), the growth differentiation factor 15 (GDF-15) influencing cardiac remodeling and apoptosis, and the 2 pro-apoptotic molecules soluble apoptosis-stimulating fragment (sFAS) and soluble tumor necrosis factor-related apoptosis-inducing ligand (sTRAIL). This multi-biomarker score had strong discriminatory power for 5-year mortality (area under the Receiver Operating Characteristic curve [AUC]=0.81) and improved risk prediction beyond the prognostic power of a comprehensive conventional risk score including known clinical predictors and NT-proBNP (AUC=0.77). Net reclassification confirmed a significant improvement of individual risk prediction (p=0.003).

Conclusions: Risk prediction by a multi-biomarker score is superior to a conventional risk score including clinical parameters and NT-proBNP. Additional predictive information from different biological pathways reflects the multisystemic character of HF.

Keywords: Biomarker; Cytokine; Heart failure; Multimarker; Prognosis; Survival.

Publication types

  • Comparative Study
  • Multicenter Study
  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Biomarkers / blood
  • Chemokine CX3CL1 / blood
  • Cohort Studies
  • Female
  • Follow-Up Studies
  • Growth Differentiation Factor 15 / blood
  • Heart Failure / blood*
  • Heart Failure / diagnosis
  • Heart Failure / mortality*
  • Hepatocyte Growth Factor / blood
  • Humans
  • Male
  • Middle Aged
  • Mortality / trends
  • Natriuretic Peptide, Brain / blood*
  • Peptide Fragments / blood*
  • Predictive Value of Tests
  • Prospective Studies
  • Risk Factors
  • TNF-Related Apoptosis-Inducing Ligand / blood
  • Time Factors

Substances

  • Biomarkers
  • CX3CL1 protein, human
  • Chemokine CX3CL1
  • GDF15 protein, human
  • Growth Differentiation Factor 15
  • Peptide Fragments
  • TNF-Related Apoptosis-Inducing Ligand
  • TNFSF10 protein, human
  • pro-brain natriuretic peptide (1-76)
  • Natriuretic Peptide, Brain
  • Hepatocyte Growth Factor