Degradable terpolymers with alkyl side chains demonstrate enhanced gene delivery potency and nanoparticle stability

Adv Mater. 2013 Mar 13;25(10):1487-93. doi: 10.1002/adma.201204346. Epub 2013 Jan 4.

Abstract

Degradable, cationic poly(β-amino ester)s (PBAEs) with alkyl side chains are developed for non-viral gene delivery. Nanoparticles formed from these PBAE terpolymers exhibit significantly enhanced DNA transfection potency and resistance to aggregation. These hydrophobic PBAE terpolymers, but not PBAEs lacking alkyl side chains, support interaction with PEG-lipid conjugates, facilitating their functionalization with shielding and targeting moieties and accelerating the in vivo translation of these materials.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Survival / drug effects
  • DNA / chemistry
  • DNA / metabolism
  • HeLa Cells
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Lipids / chemistry
  • Nanoparticles / chemistry*
  • Nanoparticles / toxicity
  • Polyethylene Glycols / chemistry
  • Polyethylene Glycols / toxicity
  • Polymers / chemistry*
  • Polymers / toxicity
  • Transfection

Substances

  • Lipids
  • Polymers
  • poly(beta-amino ester)
  • Polyethylene Glycols
  • DNA