Abstract
Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Protozoan / analysis*
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Antigens, Protozoan / immunology
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Electrochemical Techniques*
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Electrodes
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Electron Transport
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Entamoeba histolytica / metabolism*
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Ferricyanides / chemistry
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Gold / chemistry
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Humans
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Immunoassay
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Saccharomyces cerevisiae / metabolism*
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Single-Chain Antibodies / genetics
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Single-Chain Antibodies / immunology*
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Single-Chain Antibodies / metabolism
Substances
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Antigens, Protozoan
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Ferricyanides
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Recombinant Proteins
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Single-Chain Antibodies
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hexacyanoferrate III
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Gold