Candidate target genes for the Saccharomyces cerevisiae transcription factor, Yap2

Folia Microbiol (Praha). 2013 Sep;58(5):403-8. doi: 10.1007/s12223-013-0224-z. Epub 2013 Jan 19.

Abstract

In Saccharomyces cerevisiae, the Yap family of basic leucine zipper (bZip) proteins contains eight members. The Yap family proteins are implicated in a variety of stress responses; among these proteins, Yap1 acts as a major regulator of oxidative stress responses. However, the functional roles of the remaining Yap family members are poorly understood. To elucidate the function of Yap2, we mined candidate target genes of Yap2 by proteomic analysis. Among the identified genes, FRM2 was previously identified as a target gene of Yap2, which confirmed the validity of our screening method. YNL134C and YDL124W were also identified as candidate Yap2 target genes. These genes were upregulated in strains overexpressing Yap2 and possess Yap2 target sequences in their promoter regions. Furthermore, chromatin immunoprecipitation assays showed that YNL134C and YDL124W have Yap2 binding motif. These data will help to elucidate the functional role of Yap2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / biosynthesis*
  • Alcohol Oxidoreductases / genetics
  • Binding Sites
  • Chromatin Immunoprecipitation
  • DNA, Fungal / genetics
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal*
  • Oxidoreductases / biosynthesis*
  • Oxidoreductases / genetics
  • Promoter Regions, Genetic
  • Protein Binding
  • Proteomics / methods
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / biosynthesis*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Transcription Factors / metabolism*

Substances

  • CAD1 protein, S cerevisiae
  • DNA, Fungal
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Oxidoreductases
  • YNL134C protein, S cerevisiae
  • Alcohol Oxidoreductases
  • YDL124W protein, S cerevisiae