Canonical Wnt signaling inhibits osteoclastogenesis independent of osteoprotegerin

J Cell Biol. 2013 Feb 18;200(4):537-49. doi: 10.1083/jcb.201207142. Epub 2013 Feb 11.

Abstract

Although Wnt signaling is considered a key regulatory pathway for bone formation, inactivation of β-catenin in osteoblasts does not affect their activity but rather causes increased osteoclastogenesis due to insufficient production of osteoprotegerin (Opg). By monitoring the expression pattern of all known genes encoding Wnt receptors in mouse tissues and bone cells we identified Frizzled 8 (Fzd8) as a candidate regulator of bone remodeling. Fzd8-deficient mice displayed osteopenia with normal bone formation and increased osteoclastogenesis, but this phenotype was not associated with impaired Wnt signaling or Opg production by osteoblasts. The deduced direct negative influence of canonical Wnt signaling on osteoclastogenesis was confirmed in vitro and through the generation of mice lacking β-catenin in the osteoclast lineage. Here, we observed increased bone resorption despite normal Opg production and a resistance to the anti-osteoclastogenic effect of Wnt3a. These results demonstrate that Fzd8 and β-catenin negatively regulate osteoclast differentiation independent of osteoblasts and that canonical Wnt signaling controls bone resorption by two different mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Resorption
  • Cell Differentiation
  • Gene Expression Profiling
  • Mice
  • Mice, Inbred C57BL
  • Osteoclasts / cytology*
  • Osteoclasts / metabolism
  • Osteogenesis / physiology
  • Osteoprotegerin / metabolism
  • Osteoprotegerin / physiology*
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism
  • Receptors, G-Protein-Coupled / physiology
  • Receptors, Wnt / genetics
  • Receptors, Wnt / metabolism
  • Wnt Signaling Pathway / physiology*
  • Wnt3A Protein / genetics
  • Wnt3A Protein / metabolism
  • Wnt3A Protein / physiology
  • beta Catenin / genetics
  • beta Catenin / metabolism
  • beta Catenin / physiology

Substances

  • Osteoprotegerin
  • Receptors, G-Protein-Coupled
  • Receptors, Wnt
  • Wnt3A Protein
  • Wnt3a protein, mouse
  • beta Catenin
  • frizzled 8 protein, mouse