Abstract
Primary intraocular lymphoma (PIOL), also called primary vitreoretinal lymphomas, often masquerades as uveitis. This misdiagnosis can result in subsequent brain involvement and oculocerebral lymphoma (OCL). In this study, we sought to characterize the helper T-cell type 1 (Th1)/Th2 cytokine profile in vitreous samples from patients with PIOL, OCL, uveitis and controls with non-inflammatory disease. Vitreous and aqueous humor samples from 87 patients with PIOL (n = 30), OCL (n = 12), uveitis (n = 34), and retinal detachment (RD) without hemorrhage (n = 11) were analyzed and their concentrations of interleukin (IL)-2, IL-4, IL-6, IL-10, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were determined by flow cytometric bead arrays (CBA). The IL-10 levels determined by CBA were compared with those by ELISA. IL-10 concentrations measured by CBA and ELISA were highly correlated. IL-2, IL-4, and TNFα were not detected in any sample. The only cytokine detected at a significant level in samples from RD vitreous was IL-6. The IL-10/IL-6 ratio, as previously reported, was slightly higher in PIOL than in uveitis samples, but not for all patients. Cytokine profiles from PIOL and OCL samples did not differ. The combination of the IL-10/IL-6 and IL-10/IFNγ ratios was highly informative for discriminating PIOL/OCL from uveitis samples and for therapeutic follow up of PIOL. This strategy might be very helpful as an initial screening to rule out PIOL in patients thought to have uveitis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Aqueous Humor / immunology
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Aqueous Humor / metabolism
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Child
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Cytokines / immunology
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Cytokines / metabolism*
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Diagnosis, Differential
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Eye Neoplasms / diagnosis*
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Eye Neoplasms / immunology
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Eye Neoplasms / metabolism*
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Female
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Humans
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Interferon-gamma / immunology
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Interferon-gamma / metabolism
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Interleukins / immunology
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Interleukins / metabolism
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Lymphoma / diagnosis*
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Lymphoma / immunology
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Lymphoma / metabolism*
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Male
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Middle Aged
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T-Lymphocytes, Helper-Inducer / immunology
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T-Lymphocytes, Helper-Inducer / metabolism
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Th1 Cells / immunology
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Th1 Cells / metabolism
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Th2 Cells / immunology
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Th2 Cells / metabolism
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Tumor Necrosis Factor-alpha / immunology
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Tumor Necrosis Factor-alpha / metabolism
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Uveitis / diagnosis*
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Uveitis / immunology
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Uveitis / metabolism*
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Young Adult
Substances
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Cytokines
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Interleukins
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Tumor Necrosis Factor-alpha
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Interferon-gamma
Grants and funding
This work was supported by the Institut National du Cancer (Grants RC013-C06N631-2005 and C06N748-2006), the Association pour la Recherche contre le Cancer, the Institut National de la Santé et de la Recherche Médicale (INSERM), the Université Pierre and Marie Curie, the Université Paris-Descartes, the pole de compétitivité Ile de France (ImmuCan project), the Tunisian Direction Générale de la Recherche Scientifique, and the French-Tunisian CMCU “(Egide-PHC Utique)” project. R.B.A. was a recipient of grants from the DGRS-INSERM and the CMCU. S.D. was recipient of a grant from the Institut National du Cancer. V.T. was a beneficiary of study grants from the Fédération des Aveugles de France and the Fondation de France (Fouassier). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.