Effect of calcitriol on bone turnover and osteocalcin in recent-onset type 1 diabetes

PLoS One. 2013;8(2):e56488. doi: 10.1371/journal.pone.0056488. Epub 2013 Feb 20.

Abstract

Background: Vitamin D supplementation in childhood improves the achievement of peak bone mass. We investigated the effect of supplementation with calcitriol on bone turnover in recent-onset type 1 diabetes (T1D). Moreover, the association between osteocalcin and parameters of β-cell function and metabolic control was examined.

Methodology/principal findings: We conducted a post-hoc analysis of a double-blind, placebo-controlled study of calcitriol supplementation to preserve β-cell function. 27 recent-onset T1D subjects, mean age 22 years, were randomized to 0.25 µg calcitriol per day or placebo (1:1) and followed up for one year. Changes in bone formation (osteoclacin) and resorption (beta-CrossLaps) markers, and differences between placebo and calcitriol-treated group were evaluated. At baseline, osteocalcin levels were significantly lower in female than in male patients (P<0.01) while no other metabolic parameters as HbA1c and C-peptide differed between gender. No significant correlations were found in relation to HbA1c, insulin requirement and C-peptide. At 1 year follow-up, no significant differences were observed between calcitriol and placebo groups for osteocalcin and β-CrossLaps. In the placebo group osteocalcin levels were unrelated with parameters of metabolic control, such as C-peptide, insulin requirement or HbA1c. Changes of C-peptide, insulin requirement and HbA1c were not related to osteocalcin levels.

Conclusions: Supplementation with 0.25 µg calcitriol per day to patients with new-onset T1D does not affect circulating markers of bone turnover. OC levels were unrelated to β-cell function and other metabolic parameters suggesting that OC is ineffective to control pancreatic function in presence of aggressive autoimmune destruction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Age of Onset
  • Bone Resorption / complications
  • Bone Resorption / drug therapy*
  • Bone Resorption / metabolism
  • C-Peptide / blood
  • Calcitriol / administration & dosage*
  • Child
  • Collagen / blood
  • Diabetes Mellitus, Type 1 / complications
  • Diabetes Mellitus, Type 1 / metabolism*
  • Diabetes Mellitus, Type 1 / physiopathology
  • Female
  • Follow-Up Studies
  • Glycated Hemoglobin / metabolism
  • Humans
  • Insulin / blood
  • Insulin-Secreting Cells / drug effects
  • Male
  • Osteocalcin / metabolism
  • Osteogenesis / drug effects*
  • Peptide Fragments / blood
  • Vitamin D / metabolism*

Substances

  • C-Peptide
  • Glycated Hemoglobin A
  • Insulin
  • Peptide Fragments
  • glutamyl-lysyl-alanyl-histidyl-aspartyl-glycyl-glycyl-arginine
  • hemoglobin A1c protein, human
  • Osteocalcin
  • Vitamin D
  • Collagen
  • Calcitriol

Grants and funding

This study was supported by a grant from Ministero Università e Ricerca Scientifica (Progetti di Interesse Nazionale PRIN) to Paolo Pozzilli, P.I. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.