Engineered iron-oxide-based nanoparticles as enhanced T1 contrast agents for efficient tumor imaging

ACS Nano. 2013 Apr 23;7(4):3287-96. doi: 10.1021/nn305991e. Epub 2013 Mar 14.

Abstract

We report the design and synthesis of small-sized zwitterion-coated gadolinium-embedded iron oxide (GdIO) nanoparticles, which exhibit a strong T1 contrast effect for tumor imaging through enhanced permeation and retention effect and the ability to clear out of the body in living subjects. The combination of spin-canting effects and the collection of gadolinium species within small-sized GdIO nanoparticles led to a significantly enhanced T1 contrast effect. For example, GdIO nanoparticles with a diameter of ∼4.8 nm exhibited a high r1 relaxivity of 7.85 mM(-1)·S(-1) and a low r2/r1 ratio of 5.24. After being coated with zwitterionic dopamine sulfonate molecules, the 4.8 nm GdIO nanoparticles showed a steady hydrodynamic diameter (∼5.2 nm) in both PBS buffer and fetal bovine serum solution, indicating a low nonspecific protein absorption. This study provides a valuable strategy for the design of highly sensitive iron-oxide-based T1 contrast agents with relatively long circulation half-lives (∼50 min), efficient tumor passive targeting (SKOV3, human ovarian cancer xenograft tumor as a model), and the possibility of rapid renal clearance after tumor imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Contrast Media / chemical synthesis*
  • Female
  • Gadolinium* / chemistry
  • Humans
  • Magnetic Resonance Imaging / methods*
  • Magnetite Nanoparticles* / chemistry
  • Mice
  • Nanocapsules* / chemistry
  • Ovarian Neoplasms / pathology*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Contrast Media
  • Magnetite Nanoparticles
  • Nanocapsules
  • Gadolinium