Abstract
Oxidative stress is associated with age-related reactions. The anti-oxidative effects of a reduced form of co-enzyme Q10 (rCoQ10) suppress oxidative stress, which may contribute to the prevention of age-related inflammatory reactions. We examined the effects of topically applied rCoQ10 on periodontal inflammatory reactions in a rat aging model. Male Fischer 344 rats, 2 (n = 6) and 4 mos (n = 18) of age, were used. All of the two-month-old rats and 6 of the four-month-old rats were sacrificed and 12 remaining four-month-old rats received topically applied ointment with or without 1% rCoQ10 on the gingival surface until they reached 6 mos of age. The rats showed an age-dependent increase in circulating oxidative stress. RCoQ10 decreased oxidative DNA damage and tartrate-resistant acid-phosphatase-positive osteoclasts in the periodontal tissue at 6 mos of age as compared with the control. The same conditions lowered gene expression of caspase-1 and interleukin-1β in the periodontal tissue. Furthermore, Nod-like receptor protein 3 inflammasomes were less activated in periodontal tissues from rCoQ10-treated rats as compared with the control rats. Our results suggest that rCoQ10 suppresses age-related inflammatory reactions and osteoclast differentiation by inhibiting oxidative stress.
Keywords:
aging; coenzyme Q10; inflammasomes; oxidative stress; periodontium; preclinical drug evaluation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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8-Hydroxy-2'-Deoxyguanosine
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Acid Phosphatase / analysis
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Actins / drug effects
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Age Factors
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Aging / drug effects*
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Alveolar Process / drug effects
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Alveolar Process / pathology
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Animals
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Antioxidants / pharmacology
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Apoptosis Regulatory Proteins
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CARD Signaling Adaptor Proteins
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Carrier Proteins
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Caspase 1 / drug effects
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Cytoskeletal Proteins / drug effects
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DNA Damage / drug effects
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Deoxyguanosine / analogs & derivatives
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Deoxyguanosine / analysis
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Deoxyguanosine / blood
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Electron Transport Chain Complex Proteins / pharmacology*
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Gingiva / drug effects
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Gingiva / pathology
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Inflammasomes / drug effects
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Interleukin-1beta / drug effects
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Isoenzymes / analysis
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Male
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Models, Animal
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NF-kappa B / drug effects
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NLR Family, Pyrin Domain-Containing 3 Protein
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Osteoclasts / drug effects
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Oxidative Stress / drug effects
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Periodontium / drug effects*
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Periodontium / pathology
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Random Allocation
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Rats
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Rats, Inbred F344
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Receptors, Cytoplasmic and Nuclear / drug effects
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Tartrate-Resistant Acid Phosphatase
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Ubiquinone / analogs & derivatives*
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Ubiquinone / blood
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Ubiquinone / pharmacology
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Vitamins / pharmacology*
Substances
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Actins
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Antioxidants
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Apoptosis Regulatory Proteins
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CARD Signaling Adaptor Proteins
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Carrier Proteins
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Cytoskeletal Proteins
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Electron Transport Chain Complex Proteins
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Inflammasomes
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Interleukin-1beta
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Isoenzymes
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NF-kappa B
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NLR Family, Pyrin Domain-Containing 3 Protein
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Nlrp3 protein, rat
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Pycard protein, rat
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Receptors, Cytoplasmic and Nuclear
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Vitamins
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Ubiquinone
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8-Hydroxy-2'-Deoxyguanosine
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Acid Phosphatase
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Tartrate-Resistant Acid Phosphatase
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Caspase 1
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coenzyme Q10
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Deoxyguanosine