Abstract
This study investigated the impact of sleep deprivation on the human circadian system. Plasma melatonin and cortisol levels and leukocyte expression levels of 12 genes were examined over 48 h (sleep vs. no-sleep nights) in 12 young males (mean±SD: 23±5 yrs). During one night of total sleep deprivation, BMAL1 expression was suppressed, the heat shock gene HSPA1B expression was induced, and the amplitude of the melatonin rhythm increased, whereas other high-amplitude clock gene rhythms (e.g., PER1-3, REV-ERBα) remained unaffected. These data suggest that the core clock mechanism in peripheral oscillators is compromised during acute sleep deprivation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ARNTL Transcription Factors / metabolism
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Adult
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Circadian Rhythm / genetics
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Gene Expression Profiling
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Gene Expression Regulation*
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HSP70 Heat-Shock Proteins / metabolism
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Humans
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Hydrocortisone / blood
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Leukocytes / metabolism
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Light
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Male
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Melatonin / blood*
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Nuclear Receptor Subfamily 1, Group D, Member 1 / genetics
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Nuclear Receptor Subfamily 1, Group D, Member 1 / metabolism
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Oscillometry / methods
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Oxidative Stress
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Period Circadian Proteins / genetics
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Period Circadian Proteins / metabolism*
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Radioimmunoassay
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Sleep / genetics
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Sleep Deprivation / genetics*
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Sleep Deprivation / metabolism*
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Time Factors
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Young Adult
Substances
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ARNTL Transcription Factors
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BMAL1 protein, human
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HSP70 Heat-Shock Proteins
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HSPA1B protein, human
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NR1D1 protein, human
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Nuclear Receptor Subfamily 1, Group D, Member 1
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PER1 protein, human
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PER2 protein, human
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PER3 protein, human
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Period Circadian Proteins
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Melatonin
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Hydrocortisone