Eradication of Helicobacter pylori according to 23S ribosomal RNA point mutations associated with clarithromycin resistance

J Infect Dis. 2013 Oct 1;208(7):1123-30. doi: 10.1093/infdis/jit287. Epub 2013 Jun 24.

Abstract

Background: Clarithromycin-resistant Helicobacter pylori is associated with point mutations in the 23S ribosomal RNA (rRNA) gene.

Methods: A total of 1232 patients participated and were divided into 2 control groups and 1 case group. Patients in the APC control group, which consisted of 308 randomly assigned participants, were treated with standard triple therapy, consisting of amoxicillin, rabeprazole, and clarithromycin; 308 participants in the APM control group were treated with amoxicillin, rabeprazole, and metronidazole. For the 616 participants in the case group, a test for point mutations in the 23S rRNA gene of H. pylori was conducted. A total of 218 individuals in the case group received a new tailored therapy regimen, in which amoxicillin, rabeprazole, and clarithromycin were given in the absence of a mutation, whereas clarithromycin was replaced by metronidazole if the mutation was detected.

Results: The rate of eradication of H. pylori in the tailored group was 91.2% (176/193), which was significantly higher than that in the APC (75.9% [214/282]; P < .001) and APM (79.1% [219/277]; P < .001) control groups.

Conclusion: The rate of H. pylori eradication among patients who received tailored therapy on the basis of detection of a clarithromycin resistance mutation by polymerase chain reaction was much higher than the rate among patients who received a standard triple therapy regimen.

Clinical trials registration: NCT0145303.

Keywords: Clarithromycin; Helicobacter pylori; drug resistance; point mutation.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Pyridinylmethylsulfinylbenzimidazoles / therapeutic use
  • Adult
  • Aged
  • Amoxicillin / therapeutic use
  • Anti-Bacterial Agents / pharmacology
  • Anti-Bacterial Agents / therapeutic use*
  • Clarithromycin / pharmacology
  • Clarithromycin / therapeutic use*
  • Drug Resistance, Bacterial*
  • Drug Therapy, Combination / methods
  • Female
  • Helicobacter Infections / drug therapy*
  • Helicobacter pylori / drug effects
  • Helicobacter pylori / genetics
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Male
  • Metronidazole / therapeutic use
  • Middle Aged
  • Point Mutation*
  • Polymerase Chain Reaction
  • Proton Pump Inhibitors / therapeutic use
  • RNA, Ribosomal, 23S / genetics*
  • Rabeprazole
  • Treatment Outcome
  • Young Adult

Substances

  • 2-Pyridinylmethylsulfinylbenzimidazoles
  • Anti-Bacterial Agents
  • Proton Pump Inhibitors
  • RNA, Ribosomal, 23S
  • Metronidazole
  • Rabeprazole
  • Amoxicillin
  • Clarithromycin