Performance of MALDI-ToF MS for species identification of Burkholderia cepacia complex clinical isolates

Diagn Microbiol Infect Dis. 2013 Oct;77(2):126-8. doi: 10.1016/j.diagmicrobio.2013.06.011. Epub 2013 Jul 23.

Abstract

We evaluated the performance of matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) for identification of Bcc species compared with that of recA sequencing. MALDI-ToF was able of identifying 100% of Bcc isolates at the genus level, but 23.1% of Bcc isolates tested were not correctly identified at the species level. The misidentification occurred most frequently with Burkholderia contaminans (100%) and B. cepacia (33.3%).

Keywords: Bacteria identification; Bruker; Mass spectrometry; Non-fermentative bacilli; recA gene sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques / methods*
  • Burkholderia Infections / microbiology
  • Burkholderia cepacia complex / chemistry
  • Burkholderia cepacia complex / classification*
  • Burkholderia cepacia complex / isolation & purification
  • DNA, Bacterial / genetics
  • Humans
  • Polymerase Chain Reaction
  • Rec A Recombinases / genetics
  • Sequence Analysis, DNA
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • DNA, Bacterial
  • Rec A Recombinases