Detailed analysis of inflammatory cell infiltration in colorectal cancer

Br J Cancer. 2013 Oct 1;109(7):1839-47. doi: 10.1038/bjc.2013.508. Epub 2013 Sep 5.

Abstract

Background: Higher-grade inflammatory infiltrate is a promising marker for better prognosis in colorectal cancer (CRC). However, the knowledge on the interrelationships between different inflammatory cells and classifications is fragmentary.

Methods: We analysed the densities of eight types of inflammatory cells in a prospectively recruited group of 117 CRC patients and determined their interrelationships and contributions to Klintrup-Mäkinen (K-M) score of overall peritumoural inflammation. We characterised the inflammatory infiltrate in relation to stage and recurrences in 24-month follow-up.

Results: There were high positive correlations between the inflammatory cell densities, with the exception of mast cells and CD1a+ immature dendritic cells. High K-M score associated with high peri- and intratumoural densities of CD3+, CD8+, CD68+, CD83+, and FoxP3+ cells and neutrophils. Advanced stage associated with low K-M score, as well as low CD3+, CD8+, CD83+, and FoxP3+ cell counts, of which low K-M score, low CD3(+) T-cell count, and low FoxP3+ T-cell count were linked to higher recurrence rate.

Conclusion: The density of CRC inflammatory infiltrate declines as stage advances. Especially, low K-M score and low T-cell counts predict higher recurrence rate. The high positive correlations between the individual inflammatory markers support the value of overall inflammatory reaction scoring.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Antigens, CD / metabolism
  • Antigens, CD1 / metabolism
  • Antigens, Differentiation, Myelomonocytic / metabolism
  • CD3 Complex / metabolism
  • CD8 Antigens / metabolism
  • CD83 Antigen
  • Colorectal Neoplasms / immunology*
  • Dendritic Cells / immunology
  • Female
  • Forkhead Transcription Factors / metabolism
  • Humans
  • Immunoglobulins / metabolism
  • Inflammation / immunology*
  • Lymphocyte Count
  • Male
  • Mast Cells / immunology
  • Membrane Glycoproteins / metabolism
  • Neoplasm Recurrence, Local
  • Neutrophils / immunology*
  • Prognosis
  • T-Lymphocytes / immunology*

Substances

  • Antigens, CD
  • Antigens, CD1
  • Antigens, Differentiation, Myelomonocytic
  • CD1a antigen
  • CD3 Complex
  • CD68 antigen, human
  • CD8 Antigens
  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • Immunoglobulins
  • Membrane Glycoproteins