Circulating microparticles from obstructive sleep apnea syndrome patients induce endothelin-mediated angiogenesis

Biochim Biophys Acta. 2014 Feb;1842(2):202-7. doi: 10.1016/j.bbadis.2013.11.017. Epub 2013 Nov 22.

Abstract

Microparticles are deemed true biomarkers and vectors of biological information between cells. Depending on their origin, the composition of microparticles varies and the subsequent message transported by them, such as proteins, mRNA, or miRNA, can differ. In obstructive sleep apnea syndrome (OSAS), circulating microparticles are associated with endothelial dysfunction by reducing endothelial-derived nitric oxide production. Here, we have analyzed the potential role of circulating microparticles from OSAS patients on the regulation of angiogenesis and the involved pathway. VEGF content carried by circulating microparticles from OSAS patients was increased when compared with microparticles from non-OSAS patients. Circulating microparticles from OSAS patients induced an increase of angiogenesis that was abolished in the presence of the antagonist of endothelin-1 receptor type B. In addition, endothelin-1 secretion was increased in human endothelial cells treated by OSAS microparticles. We highlight that circulating microparticles from OSAS patients can modify the secretome of endothelial cells leading to angiogenesis.

Keywords: Angiogenesis; Endothelin-1; Human endothelial cell; Microparticle; Obstructive sleep apnea syndrome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aorta / cytology
  • Apoptosis / physiology
  • Blotting, Western
  • Cell Adhesion / physiology
  • Cell-Derived Microparticles / metabolism
  • Cell-Derived Microparticles / physiology*
  • Endothelial Cells / drug effects
  • Endothelial Cells / metabolism
  • Endothelial Cells / physiology*
  • Endothelin B Receptor Antagonists
  • Endothelin-1 / metabolism*
  • Flow Cytometry
  • Humans
  • Male
  • Middle Aged
  • Neovascularization, Physiologic / drug effects
  • Neovascularization, Physiologic / physiology*
  • Oligopeptides / pharmacology
  • Piperidines / pharmacology
  • Receptor, Endothelin B / metabolism*
  • Sleep Apnea, Obstructive / blood
  • Sleep Apnea, Obstructive / physiopathology
  • Vascular Endothelial Growth Factor A / metabolism
  • Vascular Endothelial Growth Factor A / pharmacology
  • Young Adult

Substances

  • Endothelin B Receptor Antagonists
  • Endothelin-1
  • Oligopeptides
  • Piperidines
  • Receptor, Endothelin B
  • Vascular Endothelial Growth Factor A
  • BQ 788