Brd4 and JMJD6-associated anti-pause enhancers in regulation of transcriptional pause release

Cell. 2013 Dec 19;155(7):1581-1595. doi: 10.1016/j.cell.2013.10.056.

Abstract

Distal enhancers characterized by the H3K4me(1) mark play critical roles in developmental and transcriptional programs. However, potential roles of specific distal regulatory elements in regulating RNA polymerase II (Pol II) promoter-proximal pause release remain poorly investigated. Here, we report that a unique cohort of jumonji C-domain-containing protein 6 (JMJD6) and bromodomain-containing protein 4 (Brd4) cobound distal enhancers, termed anti-pause enhancers (A-PEs), regulate promoter-proximal pause release of a large subset of transcription units via long-range interactions. Brd4-dependent JMJD6 recruitment on A-PEs mediates erasure of H4R3me(2(s)), which is directly read by 7SK snRNA, and decapping/demethylation of 7SK snRNA, ensuring the dismissal of the 7SK snRNA/HEXIM inhibitory complex. The interactions of both JMJD6 and Brd4 with the P-TEFb complex permit its activation and pause release of regulated coding genes. The functions of JMJD6/ Brd4-associated dual histone and RNA demethylase activity on anti-pause enhancers have intriguing implications for these proteins in development, homeostasis, and disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins
  • Enhancer Elements, Genetic*
  • HEK293 Cells
  • Humans
  • Jumonji Domain-Containing Histone Demethylases / metabolism*
  • Nuclear Proteins / metabolism*
  • Promoter Regions, Genetic
  • RNA Polymerase II / metabolism*
  • RNA, Small Nuclear / metabolism
  • Transcription Factors / metabolism*
  • Transcription, Genetic*

Substances

  • BRD4 protein, human
  • Cell Cycle Proteins
  • Nuclear Proteins
  • RNA, Small Nuclear
  • Transcription Factors
  • JMJD6 protein, human
  • Jumonji Domain-Containing Histone Demethylases
  • RNA Polymerase II

Associated data

  • GEO/GSE51633