A new biometric tool for three-dimensional subcutaneous tumor scanning in mice

In Vivo. 2014 Jan-Feb;28(1):75-80.

Abstract

Aim: To propose an innovative methodology for the monitoring of the evolution of induced subcutaneous tumors in mice.

Materials and methods: A new 3D scanner able to measure the tumor mass volume is presented. The scanner is based on the projection of a fringe pattern onto the sample surface (structured light). The lines are diffused by the sample and then collected by a digital camera. The obtained 2D-image is treated by the scanner's software that extracts the 3D information and evaluates the sample volume.

Results: The 3D scanner has been successfully used in the measurement of subcutaneous HT-29 colorectal cancer xenografts treated with 5-fluorouracil, bevacizumab and their combination. Comparison with simple caliper measurements revealed important and significant differences between the two measurement techniques.

Conclusion: The proposed methodology is more effective than the usual approach based on caliper measurements.

Keywords: 3D scanner; Biometry; colorectal cancer; subcutaneous tumor measurements.

MeSH terms

  • Animals
  • Colorectal Neoplasms / diagnosis*
  • Colorectal Neoplasms / drug therapy
  • Colorectal Neoplasms / pathology
  • Fluorouracil / administration & dosage
  • HT29 Cells
  • Humans
  • Imaging, Three-Dimensional*
  • Mice
  • Software*
  • Xenograft Model Antitumor Assays

Substances

  • Fluorouracil