Tea polyphenols induced apoptosis of breast cancer cells by suppressing the expression of Survivin

Xuesong Chen; Yu Li; Qiushi Lin; Yan Wang; Hong Sun; Jian Wang; Guoquan Cui; Li Cai; Xiaoqun Dong

doi:10.1038/srep04416

Tea polyphenols induced apoptosis of breast cancer cells by suppressing the expression of Survivin

Sci Rep. 2014 Mar 20:4:4416. doi: 10.1038/srep04416.

Authors

Xuesong Chen¹, Yu Li², Qiushi Lin³, Yan Wang⁴, Hong Sun⁴, Jian Wang⁴, Guoquan Cui⁴, Li Cai⁵, Xiaoqun Dong⁶

Affiliations

¹ 1] Department of Internal Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang Province, China [2].
² 1] Bacteriologic Laboratory, Harbin Center for Disease Control and Prevention, Harbin, Heilongjiang Province, China [2].
³ 1] Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, The University of Rhode Island, Pharmacy Building, 7 Greenhouse Road, Kingston, RI 02881, USA [2].
⁴ Bacteriologic Laboratory, Harbin Center for Disease Control and Prevention, Harbin, Heilongjiang Province, China.
⁵ Department of Internal Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang Province, China.
⁶ Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, The University of Rhode Island, Pharmacy Building, 7 Greenhouse Road, Kingston, RI 02881, USA.

Abstract

To study the mechanism of tea polyphenols (TP)-induced apoptosis of breast cancer cells. Proliferation of MCF-7 and SK-BR-3 cells was evaluated by MTT assays. Cellular ultrastructure was examined by electron microscopy. Apoptosis was detected by TUNEL. PCNA、 Cyclin D1、 Cyclin E and Survivin expression was measured by Western blot. Cell proliferation was significantly inhibited by TP. Spindle and round cells were loosely distributed with increased particles after TP treatment. Increased cell size, frequent nuclear atypia and a collapse of apoptosis were observed. The nucleus was pushed towards one side, while the cytoplasm was rich in free ribosome. The membrane of mitochondria was thickening, and the cell apoptotic body was observed. TP treated cells experienced significantly enhanced apoptosis compared with 5-Fu treated or control groups. The expression of survivin was downregulated by TP. To conclude, TP can inhibit cell growth and induce apoptosis through downregulating the expression of survivin in breast cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents, Phytogenic / isolation & purification
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects*
Breast Neoplasms
Cell Line, Tumor
Cell Nucleus / drug effects
Cell Nucleus / ultrastructure
Cell Proliferation / drug effects
Cyclin D1 / genetics
Cyclin D1 / metabolism
Cyclin E / genetics
Cyclin E / metabolism
Dose-Response Relationship, Drug
Female
Gene Expression Regulation, Neoplastic*
Humans
Inhibitor of Apoptosis Proteins / antagonists & inhibitors*
Inhibitor of Apoptosis Proteins / genetics
Inhibitor of Apoptosis Proteins / metabolism
MCF-7 Cells
Mice
Mice, Nude
Mitochondria / drug effects
Mitochondria / ultrastructure
Polyphenols / isolation & purification
Polyphenols / pharmacology*
Proliferating Cell Nuclear Antigen / genetics
Proliferating Cell Nuclear Antigen / metabolism
Signal Transduction
Survivin
Tea / chemistry*
Tumor Burden / drug effects
Xenograft Model Antitumor Assays

Substances

Antineoplastic Agents, Phytogenic
BIRC5 protein, human
Cyclin E
Inhibitor of Apoptosis Proteins
Polyphenols
Proliferating Cell Nuclear Antigen
Survivin
Tea
Cyclin D1