Nanoscale probing the kinetics of oriented bacterial cell growth using atomic force microscopy

Peipei Chen; Luping Xu; Jing Liu; Felix J H Hol; Juan E Keymer; François Taddei; Dong Han; Ariel B Lindner

doi:10.1002/smll.201303724

Nanoscale probing the kinetics of oriented bacterial cell growth using atomic force microscopy

Small. 2014 Aug 13;10(15):3018-25. doi: 10.1002/smll.201303724. Epub 2014 Apr 6.

Authors

Peipei Chen¹, Luping Xu, Jing Liu, Felix J H Hol, Juan E Keymer, François Taddei, Dong Han, Ariel B Lindner

Affiliation

¹ Institut National de la Santé et de la Recherche Medicale, U1001; Faculty of Medicine, Paris Descartes University, 75014, Paris, France.

PMID: 24706390
DOI: 10.1002/smll.201303724

Abstract

Probing oriented bacterial cell growth on the nanoscale: A novel open-top micro-channel is developed to facilitate the AFM imaging of physically trapped but freely growing bacteria. The growth curves of individual Escherichia coli cells with nanometer resolution and their kinetic nano-mechanical properties are quantitatively measured.

Keywords: atomic force microscopy; bacteria; biochips; kinetics; oriented growth.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Enlargement
Cell Proliferation / physiology*
Cell Separation / instrumentation*
Equipment Design
Equipment Failure Analysis
Escherichia coli / growth & development*
Escherichia coli / ultrastructure*
Kinetics
Microfluidic Analytical Techniques / instrumentation*
Microscopy, Atomic Force / instrumentation*