How best to interpret mixed human papillomavirus genotypes in high-grade cervical intraepithelial neoplasia lesions

Emma T Callegari; Sepehr N Tabrizi; Jan Pyman; Marion Saville; Alyssa M Cornall; Julia M L Brotherton; Suzanne M Garland

doi:10.1016/j.vaccine.2014.05.041

How best to interpret mixed human papillomavirus genotypes in high-grade cervical intraepithelial neoplasia lesions

Vaccine. 2014 Jul 7;32(32):4082-8. doi: 10.1016/j.vaccine.2014.05.041. Epub 2014 May 22.

Authors

Emma T Callegari¹, Sepehr N Tabrizi², Jan Pyman³, Marion Saville⁴, Alyssa M Cornall⁵, Julia M L Brotherton⁶, Suzanne M Garland⁷

Affiliations

¹ The University of Melbourne, Grattan Street, Parkville, Melbourne 3010, VIC, Australia; Murdoch Childrens Research Institute, Parkville, VIC, Australia. Electronic address: [email protected].
² Regional HPV Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, VIC, Australia; Murdoch Childrens Research Institute, Parkville, VIC, Australia; Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, VIC, Australia. Electronic address: [email protected].
³ Department of Anatomical Pathology, Royal Women's Hospital, 5th Floor, 20 Flemington Road, Parkville, Melbourne 3052, VIC, Australia. Electronic address: [email protected].
⁴ VCS Incorporated, 265 Faraday St, Carlton, Melbourne 3053, VIC, Australia. Electronic address: [email protected].
⁵ Regional HPV Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, VIC, Australia; Murdoch Childrens Research Institute, Parkville, VIC, Australia. Electronic address: [email protected].
⁶ VCS Incorporated, 265 Faraday St, Carlton, Melbourne 3053, VIC, Australia; School of Population and Global Health, University of Melbourne, Melbourne, VIC, Australia. Electronic address: [email protected].
⁷ Regional HPV Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, VIC, Australia; Murdoch Childrens Research Institute, Parkville, VIC, Australia; Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, VIC, Australia. Electronic address: [email protected].

PMID: 24857693
DOI: 10.1016/j.vaccine.2014.05.041

Abstract

Objectives: This study aimed to determine human papillomavirus (HPV) genotypes present in biopsy sections from young women of vaccine eligible age living in Victoria, Australia, with confirmed cervical intraepithelial neoplasia grade 3 (CIN3) or adenocarcinoma in situ (AIS) using laser capture microdissection (LCM).

Methods: Histologically confirmed CIN3 or AIS positive biopsies from vaccine eligible women (born after 30th June 1981, n=169), between May 2011 and March 2013, were identified. CIN3 or AIS lesions were isolated from biopsy material using LCM, and the HPV genotypes present in whole tissue sections (WTS) as well as LCM-isolated lesion tissue were determined by a sensitive reverse hybridisation assay; RHA kit HPV SPF10-LiPA25, version 1 (Labo Bio-medical Products, Rijswijk, The Netherlands).

Results: One hundred and sixty-eight cases were shown to be HPV positive (99%), of which 20 (12%) had more than one HPV genotype detected using WTS-PCR. Evaluation by LCM of individual biopsies with mixed infections showed 18 cases (90%) had only one HPV genotype associated with each CIN3 lesion. HPV 16 was the most common HPV type, found in 95/168 cases (57%).

Conclusion: LCM-PCR allowed us to confirm the presence of a single HPV genotype associated with each biologically separate CIN3 lesion, supporting the theory that only one virus type causes each independent CIN lesion. LCM will provide an important tool in assessing vaccine effectiveness in HPV vaccine programs.

Keywords: AIS; CIN3; Cervical cancer; Human papillomavirus; Laser capture microdissection; Multiple infections.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma in Situ / virology*
Adolescent
Adult
Australien
DNA, Viral / isolation & purification
Female
Genotype
Humans
Laser Capture Microdissection
Papillomaviridae / classification*
Papillomavirus Infections / virology*
Uterine Cervical Dysplasia / virology*
Young Adult

Substances

DNA, Viral