Observation of correlated X-ray scattering at atomic resolution

Philos Trans R Soc Lond B Biol Sci. 2014 Jul 17;369(1647):20130315. doi: 10.1098/rstb.2013.0315.

Abstract

Tools to study disordered systems with local structural order, such as proteins in solution, remain limited. Such understanding is essential for e.g. rational drug design. Correlated X-ray scattering (CXS) has recently attracted new interest as a way to leverage next-generation light sources to study such disordered matter. The CXS experiment measures angular correlations of the intensity caused by the scattering of X-rays from an ensemble of identical particles, with disordered orientation and position. Averaging over 15 496 snapshot images obtained by exposing a sample of silver nanoparticles in solution to a micro-focused synchrotron radiation beam, we report on experimental efforts to obtain CXS signal from an ensemble in three dimensions. A correlation function was measured at wide angles corresponding to atomic resolution that matches theoretical predictions. These preliminary results suggest that other CXS experiments on disordered ensembles--such as proteins in solution--may be feasible in the future.

Keywords: Bragg peak correlations; X-ray angular correlations; atomic resolution X-ray scattering; silver nanoparticles; solution ensemble; synchrotron radiation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Electrons*
  • Lasers*
  • Metal Nanoparticles / chemistry
  • Models, Theoretical
  • Molecular Conformation*
  • Scattering, Radiation*
  • Silver / chemistry
  • X-Ray Diffraction / instrumentation*
  • X-Ray Diffraction / methods*

Substances

  • Silver