Up-regulation of microRNA-145 associates with lymph node metastasis in colorectal cancer

Wei Yuan; Chenguang Sui; Qian Liu; Wanyan Tang; Huaying An; Jie Ma

doi:10.1371/journal.pone.0102017

Up-regulation of microRNA-145 associates with lymph node metastasis in colorectal cancer

PLoS One. 2014 Jul 14;9(7):e102017. doi: 10.1371/journal.pone.0102017. eCollection 2014.

Authors

Wei Yuan¹, Chenguang Sui¹, Qian Liu², Wanyan Tang¹, Huaying An¹, Jie Ma¹

Affiliations

¹ State Key Laboratory of Molecular Oncology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
² Department of Abdominal Surgical Oncology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

Abstract

Metastasis is the main cause of mortality in patients with solid tumours. Identifying the exact molecules associated with CRC metastasis may be crucial to understand the process, which might also be translated to the diagnosis and treatment of CRC. In this study, we investigate the association of microRNA expression patterns with the lymph node metastasis of colorectal cancer. Among these candidate miRNAs, the expression of miRNA-145 was significantly related to lymph node metastasis of CRC. Both in vitro and in vivo study demonstrated that up-regulation of miR-145 could improve the ability of migration and invasion of colorectal cancer cell, while no effect on proliferation was observed. The mechanism of this promotion is associated with the stabilization of Hsp-27, a protein which plays an important role in the promotion of metastasis. These results may be crucial to understanding CRC metastasis and may be translated to the diagnosis and treatment of CRC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers, Tumor / metabolism
Cell Movement / drug effects
Colorectal Neoplasms / physiopathology*
Gene Expression Profiling
Gene Expression Regulation, Neoplastic / physiology*
HSP27 Heat-Shock Proteins / metabolism
Heat-Shock Proteins
Humans
Lymphatic Metastasis / physiopathology*
MicroRNAs / metabolism*
MicroRNAs / pharmacology
Molecular Chaperones
Neoplasm Invasiveness / physiopathology
Real-Time Polymerase Chain Reaction

Substances

Biomarkers, Tumor
HSP27 Heat-Shock Proteins
HSPB1 protein, human
Heat-Shock Proteins
MIRN145 microRNA, human
MicroRNAs
Molecular Chaperones

Grants and funding

This study was supported by the National Basic Research Program of China (Grant no. 2011CB911004), The Beijing Training Project for The Leading Talents (Z131107000513001), Beijing Nova Program (Z131107000413066) and Beijing Natural Science Foundation of China (Grant no. 7122150). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.